Protective Effect of Tricholoma Matsutake Extract on Alcoholic Liver Disease Based on Zebrafish Model

Abstract

In China, average alcohol intake and alcoholic liver disease (ALD) incidence have demonstrated escalating trends, yet the diagnostic and therapeutic infrastructure for ALD remains underdeveloped. At present, there are still some problems in the therapeutic drugs of alcoholic liver disease, such as poor therapeutic effect, obvious side effects and use restrictions. Therefore, there is a continuous need to develop new, safer, and more effective therapeutic drugs to meet clinical demands. This study, based on a zebrafish adult model of chronic ALD, the protective effects of Tricholoma matsutake (T. matsutake) extract were evaluated, and the potential mechanisms were explored. Results revealed that the extract significantly alleviated ethanol-induced hepatic lipid accumulation, restored liver architecture, normalized liver function indices, and reversed oxidative stress perturbations in adult zebrafish. Through transcriptome sequencing, the bioinformatics analysis demonstrated that T. matsutake extract exerted hepatoprotective effects against ALD by modulating key genes involved in lipid metabolism, glucose homeostasis, acetaldehyde detoxification, and oxidative stress responses. This study, based on zebrafish model, elucidated the hepatoprotective effects of T. matsutake extract against ALD, offering novel therapeutic strategies for its clinical management.